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GLP-1 was measured with a Glucagon-Like Peptide-1 (Active) ELISA kit (Millipore), and ghrelin in an automated enzyme immunoassay system (AIA-600 II Immunoassay Analyzer, TOSOH Bioscience)

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The Fc fragment is modified to reduce Fc receptor binding affinity and immunogenic potential [16]

The formula is always: dose divided by concentration, times 100