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J Allergy Clin Immunol 118 : 12181225 Fitzpatrick AM, Teague WG, Holguin F, Yeh M, Brown LA, Severe Asthma Research Program 2009 Airway glutathione homeostasis is altered in children with severe asthma: evidence for oxidant stress

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ImageStream sample preparation and processing Immune cells freshly isolated from the kidney marrow were fixed for 30 min in 4% PFA, then centrifuged at 400 g for 10 min and washed once in dPBS1X

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