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Luciferase activity was quantified using a dual-luciferase assay system (Promega, E1910)

Microstructure and volume No substantial differences emerged in the microstructure of white matter (WM) pathways (mean FA difference [95%-CI]: 0.001 [ 0.0140.011], P = 0.815), gray matter (GM) in the target region (mean MD difference [95%-CI]: 5.3e 05 [ 1.2e 042.3e 05], P = 0.163), or volumes of WM tracts (mean difference [95%-CI]: 15.6 [ 397.5366.2], P = 0.933) and GM targets (mean difference [95%-CI] 1.1e 04 [ 1.9e 044.2e 04], P = 0.460) after the intervention between the anodal and sham stimulation groups (see Additional file 1: Table S2)

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6 Synergic anticancer effect of sulforaphane in combination with other chemotherapeutic agents Sulforaphane can also be used with conventional cancer treatments to achieve an enhanced effect

Acupuncture Acupuncture involves the stimulation of specific acu-points, or meridian points, on the skin to normalize aberrant flow of Qi by the insertion of fine needles either by hand or through electrical stimulation