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PMID: 309 GaoJAksoyBADogrusozUDresdnerGGrossBSumerSOet al

Preparation of whole cell lysates and analysis by western blotting HCT116 dTAG-SNIP1 cells were plated onto 10 cm plates to reach 50% confluency at the day of experiment

Moreover, NK cells transfected with small interfering RNA (siRNA) against PRF1 showed dramatic decreases in PRF1 expression, coupled with downregulated NK cell cytotoxicity (data not shown), suggesting that perforin is an essential factor for determining NK cell killing capacity

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