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As expected, 0.1% BSA and 1% HSA reduced the potency of the lipidated peptides (elorilintide and cagrilintide) at human AMY1R, AMY3R, and CTR, while the non-lipidated rat amylin and sCT showed no significant potency shifts

The 5 and 3 targeting regions were independently cloned into the TOPO TA vector (Invitrogen TM ), digested and cloned into the pL0034-digested plasmid

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