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After 48 h, the cells were washed with PBS (Gibco) and incubated in the assay buffer (HBSS supplemented with Ca 2+ and Mg 2+ (Gibco), 10 mM HEPES (Gibco), 0.1% Pluronic F-68 (Gibco) and 500 M IBMX (Sigma), pH 7.4) with different concentrations of GLP-1 (0 M to 1 M) in the presence (100 nM and 1000 nM) or absence of Fab7F38 at 37 C for 30 min

however, the specific mechanism of action needs to be studied in depth (166)

Nutrient Levels to Consider During GLP-1 Weight Loss Significant appetite reduction and rapid weight loss can affect nutrient intake over time

Physiological roles of the GIP receptor in murine brown adipose tissue