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Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA

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Interestingly rats given p53 inhibitors and amifostine did not have improved survival compared to controls, suggesting amifostine acts (at least in part) via a p53 dependent mechanism [55]