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d , Immunoblot analysis of FSP1 and VCP in parental as well as HT1080 FSP1-overexpressing and FSP1 -knockout HT1080 cells

10 nM) were added to port A, 22 l oligomycin (O4876

Taken together, these results demonstrate that FHA domain residues mediating interaction with P-SF3B1 are essential for efficient pre-mRNA splicing and cell viability

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The residue was collected by centrifugation at 14,200 g at 4 C