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A 3 mL of reaction mixture was prepared with 0.1 mL of 13 mM L-methionine, 0.1 mL of 75 M p - nitroblue terazolium chloride (NBT), 0.1 mL of 100 M EDTA, 0.1 mL riboflavin (2 M) in a 1.5 mL of 50 mM potassium phosphate buffer pH 7.8, 50 L of the enzymatic extract and distilled water

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