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The supernatant was carefully extracted, and the pellet was resuspended in 1.5 mL of cold 1x PBS and transferred to 2 mL polypropylene tubes (Product No: 357324) into a pre-cooled adaptor for the Beckman Coulter 55 Ti Swinging Bucket Rotor for 2 h at 131,981 x g in the Beckman Coulter L-80 Optima Ultracentrifuge set to 4 C