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Qualified RNA was reverse transcribed into double-stranded cDNA and was sequenced for 2 150-bp runs (paired-end) using a Hiseq 2000 sequencing system (Illumina, USA), this being performed at Bioacme Biological Technologies Corporation (Wuhan, China)

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GHK-Cu peptide (Copper Tripeptide-1) is a synthetic tripeptide-copper complex referenced in peptide chemistry, molecular biology, and biochemical research literature